Primer volume
Forward and reverse primer stock volumes per reaction.
What this calculator does
Calculate forward and reverse primer stock volumes per PCR reaction from stock and final concentrations with C1V1 = C2V2 and optional setpoint checks.
Calculation model
C₁·V₁ = C₂·V₂ applied per primer + setpoint audit
Formulas
- F15 · PCR primer stock volume:
V_primer = C_primer,final · V_reaction / C_primer,stock - P08 · Instrument setpoint quantization:
setpoint_nL = round(V · 1000 / increment_nL) · increment_nL (nearest | down | up);settable ⇔ range_min ≤ setpoint ≤ range_max; increment ≠ accuracy
Assumptions
- Volumes are additive and solutions homogeneous.
- Primer stock concentration is the actual measured/specified value.
Verification status
- No recommended primer concentration is implied; enter the value from your protocol.
- tests-passed means automated algorithm tests (unit, property, integration and browser end-to-end) pass. It is not an independent scientific review and not laboratory validation.
References
- [S05] NEB: PCR Using Q5 High-Fidelity 2X Master Mix (M0492) (accessed 2026-10-29)
- [EP01] Eppendorf: Research plus technical data (DE): ranges, increments, published test-point deviations. Pinned dataset; live page not re-verified (HTTP 403, 2026-10-29) (accessed 2026-10-29)
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