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Calculators

Quick calculations

• Stock dilution: Stock and diluent volumes from C₁V₁ = C₂V₂.
• Serial dilution: Dilution series with per-tube volumes and concentrations.

DNA

• dsDNA mass & molarity: ng ↔ pmol, ng/µL ↔ nM, copy number, mass ↔ volume.
• Sequence basics: Length, base counts, GC content and reverse complement.

PCR

• PCR master mix: Per-reaction and batch volumes, custom or kit example preset.
• Primer volume: Forward and reverse primer stock volumes per reaction.

Cloning & assembly

• DNA ligation: Insert mass and pipetting volumes for an insert:vector molar ratio.

Reagents

• Molarity & mass: Mass, concentration, volume or amount from molecular weight.
• Reaction / master mix: Generic reaction setup with water to volume and batch scaling.